Book Review: Long-Term Bone Marrow Culture

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Long-term culture of human bone marrow cells.

A method has been described for the long-term culture of human bone marrow cells in liquid medium. Hematopoiesis, as measured by the production of granulocytic-macrophage progenitor cells (CFUc), continued for at least 20 weeks and was dependent upon the presence of a marrow-derived adherent layer of cells. As in the case of murine marrow liquid cultures, the adherent layer consisted of mononuc...

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Long-term culture of normal and leukemic human bone marrow.

Clonal culture systems using semisolid media for the detection of granulocyte-macrophage (Bradley and Metcalf 1966; Pike and Robinson 1970; Pluznik and Sachs 1966), erythroid (Stephenson et al. 1971), lymphoid (Fibach et al. 1976; Metcalf et al. 1975a; Sredni et al. 1976) and megakaryocytic (Metcalf et al. 1975b) progenitors have contributed greatly to investigations of hematopoiesis. Unfortuna...

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Enriched for Long-Term Bone Marrow Culture Initiating Cells

http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#repub_requests Information about reproducing this article in parts or in its entirety may be found online at: http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#reprints Information about ordering reprints may be found online at: http://bloodjournal.hematologylibrary.org/site/subscriptions/index.xhtml Information abo...

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RAPID COMMUNICATION Long-Term Bone Marrow Culture in Persons With Fanconi Anemia and Bone Marrow Failure

Fanconi anemia is an autosomal recessive disease characterized by a high risk of developing bone marrow (BM) failure and acute myelogenous leukemia. We studied growth of hematopoietic progenitor cells in long-term BM culture (LTBMC) in 8 persons with Fanconi anemia and BM failure. Although LTBMC were initiated with very few BM cells, an adherent layer formed in cultures from 7 persons. In these...

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Long-term bone marrow culture in persons with Fanconi anemia and bone marrow failure.

Fanconi anemia is an autosomal recessive disease characterized by a high risk of developing bone marrow (BM) failure and acute myelogenous leukemia. We studied growth of hematopoietic progenitor cells in long-term BM culture (LTBMC) in 8 persons with Fanconi anemia and BM failure. Although LTBMC were initiated with very few BM cells, an adherent layer formed in cultures from 7 persons. In these...

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ژورنال

عنوان ژورنال: Journal of the Royal Society of Medicine

سال: 1986

ISSN: 0141-0768,1758-1095

DOI: 10.1177/014107688607900930